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Specifications
| Appearance: | White to off-white powder |
| Solubility Clarity: | 0.2 M solution in acetonitrile is free from undissolved particulate |
| Identity (by HNMR): | To conform structure |
| Purity (HPLC): | 99.0% min |
| Single critical impurity: | 0.3% max |
| Non-critical single impurity: | 0.5% max |
| Unknown single impurity: | 0.3% max |
| 31P NMR Purity: | 98.0% min |
| P(II) Impurities (100–170 ppm): | 0.5% max |
| Residual Solvent: | 3.0% max |
| Moisture: | 0.5% max |
Applications
1. Synthesis of 2′-Fluoro-Modified Oligonucleotides
Enables incorporation of 2′-fluoro-deoxyadenosine into DNA or RNA strands using solid-phase synthesis.
Forms part of fully or partially modified oligonucleotides for enhanced stability and performance.
2. RNA Therapeutics
Used in siRNA, antisense oligonucleotides (ASOs), and aptamers to increase resistance to nucleases and improve pharmacological properties.
Enhances target affinity and binding strength while reducing immune activation.
3. Structural and Biochemical Studies
Allows exploration of RNA mimicry and RNA–protein interactions by introducing RNA-like modifications in synthetic oligonucleotides.
Useful in mapping the role of 2′-modifications in structure-function relationships of nucleic acids.
4. Diagnostic Tools
Incorporated into probe and primer designs to extend shelf life and improve hybridization stability in PCR, qPCR, and hybridization-based assays.
Benefits
✔️ High Nuclease Resistance
The 2′-fluoro group blocks degradation by RNases and DNases, increasing the stability of oligonucleotides in biological fluids.
✔️ Enhanced Duplex Stability
2′-F modifications raise melting temperature (Tm) and strengthen Watson–Crick base pairing, leading to improved binding specificity.
✔️ RNA-like Properties with DNA Backbone Stability
Ideal for constructing RNA mimics with superior pharmacokinetic properties.
✔️ Reduced Immunogenicity
2′-fluoro modifications help evade immune recognition, important in therapeutic oligonucleotide design.
✔️ Efficient Synthesis
DMT and Bz protection groups allow for high-yield, high-purity oligonucleotide assembly using standard automated synthesis protocols.
Conclusion
DMT-2′-Fluoro-dA(Bz) Amidite (CAS 136834-22-5) is a 2′-fluoro-modified deoxyadenosine building block used in the synthesis of chemically stabilized oligonucleotides. It plays a crucial role in therapeutic nucleic acid technologies, including siRNA, ASOs, and aptamers, by improving stability, target binding, and in vivo performance. Its high-quality protection and synthetic compatibility make it indispensable for modern nucleic acid drug development and molecular diagnostics.
