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Specifications
| Appearance: | White to off-white powder |
| Solubility Clarity: | 0.2 M solution in acetonitrile is free from undissolved particulate |
| Identity (by HNMR): | To conform structure |
| Purity (HPLC): | 99.0% min |
| Single critical impurity: | 0.3% max |
| Non-critical single impurity: | 0.5% max |
| Unknown single impurity: | 0.3% max |
| 31P NMR Purity: | 98.0% min |
| P(III) Impurities (100–170 ppm): | 0.5% max |
| Residual Solvent: | 3.0% max |
| Moisture: | 0.5% max |
Applications
1. Synthetic RNA Oligonucleotide Production
Used in solid-phase oligonucleotide synthesis (SPOS) to build chemically modified RNA strands.
Essential for preparing RNA containing 2′-O-methyl modifications, which are found naturally in rRNA, tRNA, and regulatory RNAs.
2. RNA Therapeutics Development
Incorporated into siRNA, mRNA, antisense oligonucleotides (ASOs), and RNA aptamers.
2′-O-methyl modifications:
Enhance resistance to nuclease degradation
Reduce activation of innate immune sensors
Improve RNA stability and pharmacokinetics in vivo
3. Epitranscriptomics and RNA Modification Research
Enables the study of the role of natural 2′-O-methylguanosine in RNA folding, translation, and recognition by proteins.
Useful for profiling and mapping RNA modifications in biological samples.
4. Biochemical and Structural Studies
Helps design structure-function experiments involving modified RNA.
Allows researchers to study RNA-protein interactions, ribozyme activity, and RNA folding dynamics with enhanced stability.
Benefits
✔️ High RNA Stability
2′-O-methyl modification provides protection against RNases, increasing RNA half-life in biological environments.
✔️ Reduced Immunogenicity
Modified RNAs are less likely to trigger immune responses, which is crucial for therapeutic use in humans.
✔️ Precise and Efficient Synthesis
The DMT, N-iBu, and CEDPA protecting groups enable high-yield, sequence-specific synthesis with excellent coupling efficiency.
✔️ Biological Relevance
Mimics naturally occurring RNA modifications, allowing the creation of biologically active and structurally accurate synthetic RNA.
Conclusion
5-O-DMT-2-O-Me-rG(N-iBu)-3-CEDPA (CAS 150780-67-9) is a protected phosphoramidite derivative of 2′-O-methylguanosine, tailored for solid-phase synthesis of modified RNA oligonucleotides. Its application spans from therapeutic RNA development (e.g., siRNA, mRNA vaccines) to biochemical and epigenetic research. By enhancing RNA stability, reducing immunogenicity, and enabling precise incorporation of RNA modifications, it plays a vital role in modern molecular biology and pharmaceutical innovation.
