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Specifications
| Appearance: | Off-white or white amorphous powder |
| Purity (HPLC): | 98% min |
| Purity (by 31P NMR): | 98% min |
| Identity (by 1H NMR): | To conform structure |
| Residual solvents: | 3.0% max |
| Water: | 0.5% max |
| Solubility Clarity: | 0.2 M solution in acetonitrile is free from undissolved particulate |
Applications
1. Synthesis of Modified RNA Oligonucleotides
Used in solid-phase chemical synthesis to incorporate 2′-O-methylcytidine with N4-acetyl protection.
Enables precise construction of RNA molecules containing natural or designed epitranscriptomic modifications.
2. RNA Therapeutics and Vaccine Development
Essential for producing modified RNA molecules (e.g., siRNA, antisense oligonucleotides, mRNA vaccines) that are more stable and less immunogenic.
2′-O-methyl and N4-acetyl modifications enhance nuclease resistance and reduce innate immune activation.
3. Epitranscriptomics Research
Allows study of how specific cytidine modifications affect RNA structure, function, and interactions.
Helps characterize the biological roles of N4-acetylation and 2′-O-methylation in RNA processing and gene regulation.
4. Molecular Biology and Biochemistry
Used in enzyme assays to analyze methyltransferase and acetyltransferase activities.
Facilitates the investigation of RNA-protein interactions and RNA stability mechanisms.
Benefits
Enhanced RNA Stability: Incorporation of 2′-O-methyl and N4-acetyl groups improves RNA resistance to degradation.
Reduced Immunogenicity: Modified nucleosides decrease immune recognition of synthetic RNA therapeutics.
High Synthesis Efficiency: Protective groups ensure precise and efficient coupling during RNA synthesis.
Research Versatility: Enables detailed studies of RNA modifications and their biological implications.
Conclusion
5-O-DMT-2-O-Me-rC(N-Ac)-3-CEDPA (CAS 199593-09-4) is a chemically protected phosphoramidite derivative of 2′-O-methylcytidine with N4-acetyl protection, essential for solid-phase RNA synthesis. It enables incorporation of biologically relevant modifications into synthetic RNA oligonucleotides used in RNA therapeutics, epitranscriptomics research, and molecular biology. This reagent facilitates the production of stable, functional, and less immunogenic RNA molecules for diverse applications in biotechnology and pharmaceutical sciences.
