N4-Acetyl-2-O-Methyl-cytidine丨CAS 113886-71-8

1. Epitranscriptomic and RNA Modification Research

Serves as a standard or reference compound to study RNA modifications, particularly N4-acetylation and 2′-O-methylation in RNA molecules.

Helps elucidate the functional roles of combined base and sugar modifications on RNA structure, stability, and protein interactions.

2. Molecular Biology and Synthetic Biology

Used in the synthesis of modified RNA oligonucleotides for:

Investigating RNA folding and dynamics

Probing enzyme recognition and activity, such as RNA methyltransferases and acetyltransferases

Studying RNA-protein interactions relevant to gene regulation

3. Therapeutic RNA Development

Incorporated into synthetic RNA therapeutics (e.g., siRNA, mRNA vaccines) to improve:

RNA stability against nucleases

Reduced immunogenicity in vivo

Enhanced pharmacokinetic properties

4. Analytical Chemistry and Diagnostics

Used as a marker in mass spectrometry and chromatography to detect and quantify RNA modifications in biological samples.

Aids in the profiling of RNA modification patterns linked to disease states or cellular responses.

1. Improved RNA Stability

The 2′-O-methyl group protects RNA from degradation by nucleases.

N4-acetylation can influence base-pairing properties and RNA structure, adding another layer of stability.

2. Modulation of RNA Function

Modifications like N4-acetyl and 2′-O-methyl influence translation, splicing, and RNA-protein binding, affecting gene expression regulation.

3. Reduced Immune Recognition

Modified nucleosides decrease the activation of innate immune sensors, making synthetic RNAs more biocompatible for therapeutic use.

4. Key to Understanding RNA Epigenetics

Studying such modifications expands knowledge of the epitranscriptome, revealing regulatory mechanisms beyond the genetic code.