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Specifications
| Appearance: | White to off-white powder |
| Solubility Clarity (in acetonitrile and 20% DMF): | 0.2 M solution is free from undissolved particulate |
| Solubility Clarity (in dichloromethane:acetonitrile 50:50): | 0.2 M solution is free from undissolved particulate |
| Identity (by HNMR): | To conform structure |
| Purity (HPLC): | 99.0% min |
| Single critical impurity: | 0.3% max |
| Non-critical single impurity: | 0.5% max |
| Unknown single impurity: | 0.3% max |
| 31P NMR Purity: | 98.0% min |
| POI Impurities (100–170 ppm): | 0.5% max |
| Residual Solvent: | 3.0% max |
| Moisture: | 0.5% max |
Applications
1. Synthetic RNA Oligonucleotide Production
Used in solid-phase oligonucleotide synthesis (SPOS) for generating RNAs containing 2′-O-methyluridine.
Commonly applied in:
siRNA duplexes
mRNA vaccines
RNA aptamers
antisense oligonucleotides (ASOs)
2. RNA Therapeutics
Facilitates the production of chemically stabilized RNAs with improved biological activity.
2′-O-methyl modifications help:
Increase resistance to ribonucleases
Enhance binding specificity
Minimize off-target effects
Reduce immunogenicity in vivo
3. Epitranscriptomic and Molecular Biology Research
Used in modified RNA synthesis to investigate the biological roles of 2′-O-methylation, particularly in rRNA, tRNA, and snRNA.
Helps model natural RNA modifications for structure-function studies.
4. Diagnostics and Probing Tools
Incorporated into RNA probes and biosensors to improve target recognition and stability in biological assays.
Benefits
1. Enhanced RNA Stability
The 2′-O-methyl group significantly increases resistance to enzymatic degradation by RNases.
2. Reduced Immune Activation
Modified nucleosides like 2′-O-Me-rU help synthetic RNA evade innate immune detection, important for therapeutic safety.
3. Improved Hybridization Specificity
2′-O-methyl modifications enhance duplex stability and improve target discrimination in antisense and siRNA applications.
4. Reliable Synthetic Performance
DMT and CEDPA protecting groups ensure high coupling efficiency and clean deprotection, enabling high-fidelity RNA synthesis.
Conclusion
5-O-DMT-2-O-Me-rU-3-CEDPA (CAS 110764-79-9) is a phosphoramidite reagent designed for precise incorporation of 2′-O-methyluridine into synthetic RNA strands. It is essential in the creation of stable, low-immunogenic, high-performance RNA oligonucleotides used in therapeutics, molecular research, and diagnostic tools. With high stability and synthesis efficiency, it plays a critical role in advancing RNA technology and epitranscriptomics.
