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Specifications
| Appearance | White crystal powder |
| Assay | 99% min |
| Water Content | 3% max |
| pH (1% aqueous) | 9.2 - 10.2 |
| Solubility (20% aqueous) | Clear, colorless |
| Ultraviolet Absorbance (0.1M in H2O) | A260nm: 0.04 max A280nm: 0.03 max |
Applications
1. Biological Buffer
PIPES disodium salt is widely used in biological and biochemical research as a Good's buffer, particularly when a stable pH near neutrality is required.
Common uses include:
Cell and tissue culture media
Enzyme assays
Protein purification workflows
Electrophoresis and Western blotting
2. Molecular Biology and Biochemistry
Ideal for buffering solutions used in:
DNA/RNA extraction and hybridization
PCR and other enzyme-based reactions
Biochemical assay development
3. Pharmaceutical and Diagnostic Formulations
Utilized in formulations of biologics, drug delivery systems, and in vitro diagnostic kits where pH stability is critical.
Benefits
✅ Near-Neutral pH Buffering
pKa of ~6.76 ensures reliable buffering within the physiological range, suitable for sensitive biological systems.
✅ Low Metal Ion Binding
Exhibits minimal chelation of metal ions (e.g., Ca²⁺, Mg²⁺), ensuring compatibility with metal-dependent enzymes and cofactors.
✅ Highly Soluble
Freely soluble in water, allowing quick and easy buffer preparation.
✅ Stable and Reproducible
Chemically stable across a range of temperatures and concentrations, offering consistent results.
✅ UV Transparent
Very low UV absorbance at 260–280 nm, making it ideal for spectrophotometric assays involving proteins or nucleic acids.
Conclusion
PIPES disodium salt (CAS 76836-02-7) is a widely trusted zwitterionic buffer designed for pH control in the slightly acidic to neutral range. Its excellent solubility, stability, and compatibility with biological systems make it indispensable in life sciences, diagnostics, pharmaceuticals, and molecular biology, where precise and interference-free buffering is essential.
