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Specifications
| Appearance | White crystals |
| Assay (dried basis) | 99% min |
| Solubility of 10% solution | Clear |
| Loss on Drying (105℃ for 4 hrs) | 15% max |
| Absorbance @260nm (10%) | 0.05% max |
| Absorbance @280nm (10%) | 0.03% max |
| pH (1%) | 8.9 to 10.1 |
Applications
1. Biological Buffering Agent
MES sodium salt is commonly used as a Good's buffer in biochemical and biological research to maintain pH stability in the slightly acidic range.
Widely applied in:
Cell culture media
Enzyme activity assays
Protein purification
Molecular biology protocols such as nucleic acid hybridization
2. Pharmaceutical and Diagnostic Formulations
Utilized in pharmaceutical formulations and in vitro diagnostic reagents that require a stable acidic pH.
3. Electrophoresis
Used in electrophoretic techniques like PAGE, where pH control in the 5.5–6.7 range is important for biomolecule separation.
Benefits
1. Effective Buffering in Slightly Acidic pH
Maintains a stable pH near 6.1, ideal for many enzymes and biological systems that require slightly acidic conditions.
2. Minimal Metal Ion Interaction
Exhibits low binding affinity to metal ions, preserving the activity of metalloenzymes and cofactors.
3. High Water Solubility
Easily dissolves in aqueous solutions, allowing simple buffer preparation.
4. Chemical and Thermal Stability
Stable under experimental conditions, ensuring reproducibility in research and production.
5. Low UV Absorbance
Does not interfere with UV detection methods used in nucleic acid and protein assays.
Conclusion
MES sodium salt (CAS 71119-23-8) is a reliable and widely used buffering agent for maintaining pH stability in the slightly acidic range of 5.5 to 6.7. Its excellent buffering capacity, low metal ion interaction, and chemical stability make it essential in biological research, pharmaceutical formulation, electrophoresis, and diagnostic applications, supporting accurate and reproducible results.

