1,3-Bis[tris(hydroxymethyl)methylamino]propane丨CAS 64431-96-5

1. Biological Buffer

Bis-TRIS propane is a dual-range zwitterionic buffer commonly used in biological, biochemical, and molecular biology research.

Key applications include:

Electrophoresis (e.g., SDS-PAGE, native PAGE)

Enzyme kinetics and protein crystallization

Cell culture and in vitro assays

2. Molecular Biology and Biochemistry

Used to maintain pH in:

Nucleic acid extraction and PCR reactions

Antibody labeling and conjugation workflows

Buffers for DNA/RNA purification and hybridization

3. Electrophoresis and Gel Systems

Preferred in high-resolution gel systems due to its stable buffering capacity across two distinct pH ranges.

4. Pharmaceutical and Diagnostic Applications

Included in formulation buffers and diagnostic kits where minimal pH fluctuation is critical for reagent performance and stability.

1. Dual Buffering Capacity

Offers two usable buffering ranges (near pH 7 and pH 9), providing flexibility for experiments requiring pH transitions or multi-step reactions.

2. High Water Solubility and Stability

Easily soluble and chemically stable, allowing for convenient buffer preparation and long shelf life.

3. Minimal Metal Ion Binding

Exhibits low affinity for divalent cations such as Mg²⁺ and Ca²⁺, ensuring compatibility with metal-dependent enzymes and reactions.

4. Low UV Absorbance

Suitable for UV-based spectrophotometric analyses (e.g., protein and nucleic acid quantification), without introducing optical interference.

5. Biocompatibility

Non-toxic and biologically inert, suitable for live cell applications, enzyme assays, and long-term biochemical reactions.

1,3-Bis[tris(hydroxymethyl)methylamino]propane (Bis-TRIS propane, CAS 64431-96-5) is a versatile and high-performance biological buffer that supports stable pH control across two key ranges. With excellent solubility, dual buffering capacity, and compatibility with sensitive biological systems, it is widely used in molecular biology, biochemistry, electrophoresis, and pharmaceutical formulations. Its low toxicity and minimal interaction with metal ions make it a reliable choice for both research and industrial applications.