![3-[(1,1-Dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic Acid丨CAS 68399-79-1](/uploads/42644/3-1-1-dimethyl-2-hydroxyethyl-amino-24d122.png)
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Specifications
| Appearance | White powder |
| Assay | 98% min |
| Loss on drying | 1.0% max |
| pH (1% aq soln) | 5.5 ~ 6.5 |
| Melting point | 215 ~ 225℃ |
| OD260 nm (0.1M aq soln) | 0.1 max |
| OD280 nm (0.1M aq soln) | 0.1 max |
Applications
1. Biological Buffering Agent
AMPSO is a zwitterionic buffering agent used in biochemistry and molecular biology.
It belongs to the class of Good's buffers, designed for minimal biological interference.
Common applications include:
Protein purification and enzyme assays
Electrophoresis buffers
Cell-free biochemical systems
2. Electrophoresis and Chromatography
Used in PAGE (polyacrylamide gel electrophoresis) and capillary electrophoresis to stabilize pH and improve separation reproducibility.
3. Cell Culture and Biomedical Research
Suitable for mammalian cell systems, especially when physiological pH maintenance is critical and CO₂-independent buffering is needed.
4. Biopharmaceutical Manufacturing
Used in the production and formulation of biologics, where precise pH control is vital during protein folding, purification, and stability studies.
Benefits
1. Excellent pH Control Near Neutral Range
AMPSO buffers efficiently between pH 6.9 and 8.3, ideal for physiological and enzymatic reactions.
2. Low UV Absorbance
Minimal interference with UV/Vis spectrophotometry, making it well-suited for nucleic acid and protein quantification assays.
3. Minimal Metal Ion Interaction
Exhibits low affinity for divalent cations like Ca²⁺ and Mg²⁺, avoiding disruption in metal-dependent biochemical systems.
4. Chemically Stable and Non-toxic
Stable across a broad temperature range and non-toxic to cells and enzymes, supporting consistent, reproducible results.
5. Water Solubility and Compatibility
High aqueous solubility allows for easy preparation and integration into various lab-scale and industrial-scale buffers.
Conclusion
3-[(1,1-Dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid (AMPSO, CAS 68399-79-1) is a reliable biological buffer that provides robust pH control near neutrality, with excellent compatibility in biochemical assays, electrophoresis, and protein purification workflows. Its zwitterionic nature, low toxicity, and high solubility make it a preferred choice in life science research and pharmaceutical development.
