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Specifications of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8
|
Appearance |
White Crystalline Powder |
|
Assay |
99.0% min |
|
Water |
1.0% max |
|
Water Solubility |
Colorless, clear, transparency |
|
pH |
3.5 to 5.0 |
|
Heavy Metal |
5 ppm max |
N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8 is a zwitterionic buffer used extensively in biochemical and molecular biology applications. It belongs to the family of Good's buffers, which are designed to have minimal interference with biological and chemical systems.
Key Applications of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8
1. Biological Buffering Agent
N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8 is primarily used as a biological buffer due to its optimal buffering capacity near physiological pH (7.4), making it suitable for:
● Cell culture media
● Protein purification
● Enzyme assays
● Electrophoresis systems
● Immunoassays (e.g., ELISA)
2. Tissue and Organ Preservation
Its compatibility with biological tissues allows TES to be used in organ perfusion and preservation solutions, helping maintain pH stability during transportation or storage.
3. Molecular Biology and Biochemistry
TES is often used in buffer systems for:
● Nucleic acid isolation and electrophoresis
● Reactions involving enzymes like restriction endonucleases or polymerases
● Bioconjugation reactions where stable pH is essential
4. Pharmaceutical Research
TES is used in formulation studies to evaluate drug stability in different pH environments and as part of drug delivery systems requiring controlled pH.
Benefits of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8
✅ Zwitterionic and Non-toxic
● Does not interfere with biochemical reactions or enzyme activities.
● Lacks absorbance in UV and visible ranges, making it ideal for spectrophotometric studies.
✅ Stable and Inert
● Chemically stable and resistant to autoclaving.
● Minimal interaction with divalent metal ions, unlike phosphate buffers.
✅ Ideal for pH-Sensitive Systems
● pKa of ~7.4 closely matches physiological pH, making it excellent for mammalian cell culture and protein work.
✅ Low Ionic Strength
● Its low ionic contribution helps maintain the integrity of ionic-sensitive biochemical systems.
Conclusion
N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid丨CAS 7365-44-8 is a high-performance biological buffer, particularly favored for enzyme assays, cell culture, and protein chemistry. Its buffering capacity near pH 7.4 and chemical inertness make it a versatile and reliable component in research and industrial biotech workflows. Whether used in basic research or pharmaceutical development, TES helps ensure pH control without interference, which is critical for the integrity of sensitive biological systems.
