MES Hydrate丨CAS 1266615-59-1

MES hydrate丨CAS 1266615-59-1 (2-(N-morpholino)ethanesulfonic acid hydrate) is a biologically active, water-soluble buffer commonly used in biological and chemical research. It is part of the morpholine-based buffer family, and its hydrate form is often used for precise pH control in biochemical applications. MES is often used in environments where the pH needs to remain stable over a range of temperatures.

1. Buffering Agent in Biological and Biochemical Studies
● Cell culture: MES hydrate is frequently used in cell culture media to maintain a constant pH during growth, especially in systems where the pH must be tightly controlled.
● Enzyme reactions: It is also used in enzyme assays where pH stability is crucial for the activity of certain enzymes.
● Protein electrophoresis: MES is used as a buffer in polymerase chain reactions (PCR) and protein gel electrophoresis to maintain stable pH conditions during separation processes.
2. pH Stabilizer in Pharmaceutical Formulations
● Formulation stability: MES is used to help maintain a consistent pH in pharmaceutical formulations, especially for injectables and oral drugs, where pH can affect the stability, solubility, and efficacy of the active pharmaceutical ingredient (API).
3. Analytical Chemistry
● Chromatography: MES is commonly employed in high-performance liquid chromatography (HPLC) buffers, ensuring the accurate separation and detection of compounds.
● Electrochemical analysis: It's utilized as a buffer in electrochemical studies, where a constant pH is essential for reproducibility and accuracy in measurements.
4. Biotechnological Applications
● Fermentation: MES hydrate丨CAS 1266615-59-1 is used in fermentation media to maintain an appropriate pH environment for the growth of microorganisms or cells.
● Enzyme and biocatalyst stabilization: It is applied to stabilize enzymes and biocatalysts in biotechnological processes, as it offers a low ionic strength buffer environment, reducing potential interference.
5. Cosmetic Formulations
● pH control: MES hydrate is used in the formulation of cosmetics and personal care products to maintain optimal pH levels for both product stability and skin compatibility.

1. Stable pH Range
MES has a pKa of approximately 6.1 (at 25°C), making it ideal for use in slightly acidic to neutral pH environments (around pH 6.0 to 7.5). This makes it particularly useful in biological and biochemical settings, where slight pH fluctuations can affect experimental outcomes.
2. Non-interfering with Biological Systems
MES hydrate is biologically inert, meaning it will not interfere with biological reactions or cellular processes, making it safe for use in cell culture media, enzyme assays, and other applications where biological integrity must be preserved.
3. Low Ionic Strength
Compared to many other buffers, MES has a relatively low ionic strength, reducing its potential interference with biological systems or complex reactions, which is particularly important for enzyme function and protein interactions.
4. Good Solubility
MES hydrate is highly water-soluble, allowing for easy and accurate preparation of buffer solutions at the required concentration without issues related to solubility or precipitation.
5. Wide Usage in Research
Due to its low toxicity and compatibility with various systems, MES hydrate丨CAS 1266615-59-1 is one of the most commonly used buffers in both biological research and industrial applications. It supports a wide range of experimental protocols in fields like genomics, proteomics, and pharmaceutical development.