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Specifications of Tetramethylbenzidine丨54827-17-7
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Property |
Specification |
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Appearance |
White or beige yellow crystalline powder |
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Assay (HPLC) |
99.0% min |
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Melting Point |
168.0-171.0 °C |
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Loss on Drying |
1.0% max |
|
Heavy Metals |
5 ppm max |
Transport Information of Tetramethylbenzidine丨54827-17-7
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Parameter |
Specification |
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UN Number |
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Class |
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Packing Group |
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H.S. Code |
2921430090303 |
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Stability & Reactivity |
Stable, but sensitive to moisture and may be sensitive to light. Incompatible with water and strong oxidants. |
|
Storage |
2 - 8 °C. Handle and store under inert gas. |
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Condition to Avoid |
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Package |
Introduction of Tetramethylbenzidine丨54827-17-7
Tetramethylbenzidine丨54827-17-7 is a chromogenic substrate widely used in biochemical assays, clinical diagnostics, and analytical chemistry. It is valued for its sensitivity, stability, and low toxicity, making it a preferred reagent in enzyme-linked immunosorbent assays (ELISA), peroxidase-based detection, and environmental testing.
Applications of Tetramethylbenzidine丨54827-17-7
1. Clinical and Diagnostic Applications
● Enzyme-Linked Immunosorbent Assay (ELISA) Substrate:
Used as a colorimetric substrate for horseradish peroxidase (HRP)-based ELISA tests.
Produces a blue color in the presence of HRP, which turns yellow upon acidification, enabling quantitative measurement of biomolecules.
● Blood Detection in Medical Testing:
Used in forensic science and medical diagnostics to detect trace amounts of blood via peroxidase activity.
2. Biochemical and Research Applications
● Western Blot and Immunohistochemistry (IHC):
Applied as a visualizing agent for detecting proteins and antibodies.
● Oxidative Stress and Enzyme Studies:
Used in research involving reactive oxygen species (ROS) and peroxidase activity.
3. Environmental and Industrial Testing
● Water and Wastewater Analysis:
Used to detect heavy metals and oxidizing agents in environmental samples.
● Food Safety and Quality Control:
Applied in contaminant detection and peroxide activity assays in food products.
Benefits of Tetramethylbenzidine丨54827-17-7
1. High Sensitivity and Specificity
● Provides clear and measurable color changes, making it ideal for quantitative assays.
2. Low Toxicity Compared to Other Chromogens
● Safer than traditional alternatives like benzidine, reducing health risks in laboratory use.
3. Versatile Across Multiple Applications
● Used in clinical, biochemical, environmental, and forensic testing.
4. Stable and Reliable in Assay Development
● Provides consistent results in enzyme detection and diagnostic testing.
Conclusion
Tetramethylbenzidine丨54827-17-7 is a widely used chromogenic substrate with key applications in diagnostics, biochemical research, and environmental testing. Its high sensitivity, low toxicity, and stability make it an essential reagent in ELISA, peroxidase-based assays, and forensic analysis.
