5-Methylcytosine Hydrochloride丨CAS 58366-64-6

5-Methylcytosine hydrochloride is primarily used in biochemical and molecular biology research as a key nucleoside derivative for studying DNA methylation and epigenetic modifications. It serves as a standard or reference compound in the analysis of methylated DNA, aiding in the development of assays for gene regulation, DNA-protein interactions, and epigenetic profiling. The compound is employed in the synthesis of modified oligonucleotides, nucleic acid analogs, and DNA-based probes to investigate methylation-dependent processes. In research settings, it is also used to explore enzymatic methylation pathways, develop methylation-sensitive detection methods, and support the design of epigenetic modulators and nucleic acid–based experimental tools. Its stable hydrochloride form makes it convenient for handling, storage, and incorporation into aqueous systems for laboratory studies.

The benefits of 5-Methylcytosine hydrochloride arise from its well-defined chemical structure and biological relevance. Its methylated cytosine unit allows for precise study of epigenetic patterns, facilitating reproducible experimental results in methylation analysis. The hydrochloride salt improves solubility and stability, enabling accurate preparation of solutions and reducing degradation during experiments. Its compatibility with standard nucleic acid chemistries allows integration into oligonucleotide synthesis and nucleoside modification protocols. Additionally, the compound provides a reliable reference for calibrating analytical methods such as chromatography, mass spectrometry, and sequencing-based detection, supporting high accuracy and sensitivity in epigenetic research.