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Specifications
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Appearance |
Off white to pale yellow green or pale pink powder |
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Purity (HPLC-a/a) |
98% min |
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Assay (HPLC-external standard method) |
98% min |
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Water content (Loss on drying) |
1% max |
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Identify (HPLC) |
Retention time of the principal peak in sample should match retention time of standard principal peak in HPLC |
Transport Information
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Parameter |
Specification |
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UN Number |
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Class |
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Packing Group |
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H.S. Code |
2933990099306 |
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Stability & Reactivity |
The product is chemically stable under standard ambient conditions. |
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Storage |
Keep container tightly closed. Store in a cool and shaded area. Keep under inert gas. |
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Condition to Avoid |
Exposure to air. |
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Package |
Manufacturing Information
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Parameter |
Specification |
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Capacity |
1.5MT/month |
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Frequency |
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Main Export Countries |
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Capacity/Batch |
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Experience |
Production since 2009 |
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Stock |
Overview
3-Aminophthalhydrazide丨CAS 521-31-3, commonly known as luminol, is a chemiluminescent compound widely recognized for its ability to emit blue light upon oxidation. This property has made it extremely valuable in various scientific and forensic applications.
Applications
1. Forensic Science
Used extensively in forensic investigations to detect trace amounts of blood at crime scenes.
When luminol comes into contact with the iron in hemoglobin, it reacts with an oxidizing agent (usually hydrogen peroxide) to produce a blue glow.
This chemiluminescent reaction helps investigators reveal blood stains that are otherwise invisible to the naked eye.
2. Analytical Chemistry
Used as a reagent for the detection of metal ions (such as iron and copper) and other oxidizing agents.
Employed in various biochemical assays to detect the presence of reactive oxygen species (ROS).
Used in enzyme-linked immunosorbent assays (ELISA) as a substrate for horseradish peroxidase (HRP) to produce chemiluminescent signals.
3. Biomedical Research
Utilized to study oxidative stress and cellular respiration by monitoring the generation of reactive oxygen species.
Used in imaging and detection assays for tracking biochemical and cellular processes.
4. Chemical Education and Demonstration
Commonly used in educational demonstrations to showcase chemiluminescence and chemical reactions.
Benefits
✅ Highly Sensitive Detection
Enables detection of trace amounts of blood or oxidizing substances, enhancing forensic and analytical accuracy.
✅ Non-Destructive Testing
The chemiluminescence reaction does not significantly damage the sample, allowing further analysis.
✅ Versatile in Applications
Used in forensic, chemical, and biomedical fields due to its unique light-emitting reaction.
✅ Easy to Use
The reaction is straightforward and rapid, providing immediate visual results.
Summary
3-Aminophthalhydrazide丨CAS 521-31-3 is a powerful chemiluminescent reagent essential in forensic science for blood detection, analytical chemistry for oxidative species detection, and biomedical research. Its ability to emit visible light upon oxidation makes it invaluable for sensitive and non-destructive testing.

