5-O-(4,4-Dimethoxytrityl)uridine丨CAS 81246-79-9

5-O-(4,4-Dimethoxytrityl)uridine is a protected nucleoside widely used in the field of oligonucleotide synthesis. Its 5′-hydroxyl group is blocked with a dimethoxytrityl (DMT) protecting group, which allows controlled stepwise assembly of RNA and DNA sequences on solid supports via phosphoramidite chemistry. This compound serves as a critical building block for automated oligonucleotide synthesis, enabling the incorporation of uridine residues at specific positions in RNA or DNA strands. It is employed in the preparation of antisense oligonucleotides, small interfering RNAs (siRNA), microRNAs, aptamers, and other modified nucleic acids used in therapeutic research, diagnostics, and molecular biology studies. In addition to its role in nucleotide assembly, 5-O-(4,4-Dimethoxytrityl)uridine is often used in the synthesis of labeled or modified nucleic acids for studying gene expression, protein–nucleic acid interactions, and RNA structural analysis.

The primary benefit of 5-O-(4,4-Dimethoxytrityl)uridine lies in its ability to enable precise, stepwise oligonucleotide synthesis. The DMT protecting group is stable under standard reaction conditions but can be selectively removed under mildly acidic conditions, allowing for controlled elongation of nucleotide chains. This stability ensures high coupling efficiency and minimizes undesired side reactions during synthesis. Additionally, the compound provides versatility for incorporation into chemically modified or functionalized oligonucleotides, supporting the development of therapeutics and research tools with enhanced stability, binding affinity, or chemical functionality. Its compatibility with automated synthesizers streamlines large-scale and reproducible oligonucleotide production. Overall, it helps researchers and manufacturers achieve high yield, sequence fidelity, and consistency in complex nucleic acid assemblies.