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Specifications
| Appearance: | Yellow to orange red powder |
| Purity: | 97% min |
Applications
1. Fluorescent Labeling of Biomolecules
Commonly used to label proteins, peptides, nucleotides, and other biomolecules via its carboxylic acid group.
Reacts with primary amines (via EDC/NHS chemistry) to form stable amide bonds.
Enables tracking and visualization in microscopy, gel electrophoresis, and western blotting.
2. Flow Cytometry and Fluorescence Microscopy
Emits bright green fluorescence (excitation ~494 nm, emission ~518 nm), ideal for cell staining and subcellular imaging.
Used to create fluorescent probes and tracers for quantifying cellular processes or molecular interactions.
3. Liposome and Nanoparticle Tracking
Incorporated into liposomes, microspheres, and nanocarriers to monitor uptake, release, or biodistribution in drug delivery research.
4. Quantitative Assays and Diagnostics
Utilized in enzyme activity assays, pH measurement, and fluorescent calibration standards.
Valuable for real-time PCR, fluorescent in situ hybridization (FISH), and biosensors.
5. DNA/RNA and Oligonucleotide Labeling
Used to label oligonucleotides for detecting hybridization events or quantifying nucleic acid sequences.
Provides stable fluorescence in a variety of buffer conditions.
Benefits
1. Reactive Carboxyl Group for Bioconjugation
Facilitates site-specific covalent labeling of biomolecules.
Stable and well-characterized amide linkage formation.
2. High Fluorescence Intensity
Offers bright and stable green fluorescence with high quantum yield, ensuring sensitive detection in biological systems.
3. Water Solubility and Biocompatibility
Soluble in aqueous buffers, suitable for live-cell applications.
Exhibits low toxicity at labeling concentrations.
4. Multiplexing Compatibility
Can be used alongside other fluorophores in multicolor imaging and flow cytometry panels.
5. Proven Performance in Commercial Assays
Extensively used in commercial diagnostic kits and research assays, with well-established protocols and detection systems.
Conclusion
5(6)-Carboxyfluorescein (CAS 72088-94-9) is a highly versatile and widely used fluorescent labeling reagent featuring a reactive carboxylic acid group for bioconjugation. Its strong green fluorescence, excellent water solubility, and proven performance make it ideal for applications in biological imaging, diagnostics, molecular assays, and drug delivery research. Its ability to form stable linkages with biomolecules allows for precise and efficient labeling in a wide range of experimental systems.
