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Specifications
| Appearance: | Light Brown Crystalline Powder |
| Purity: | 98% min |
| Solubility: | 500g/L |
| Substance/mixture: | Substance |
| Pb: | Not Detected |
| Cd: | Not Detected |
| As: | Not Detected |
| Wavelength: | 491nm |
| Fluorescent quantum yield: | Qualified |
| Extinction Coefficient: | Qualified |
| Melting/Freezing Point: | 320°C |
| Recommended Retest Period: | 1 Year |
Applications
1. Biological and Medical Imaging
Widely used as a fluorescent tracer in microscopy, flow cytometry, and cell biology to label cells, proteins, and nucleic acids.
Used in angiography to visualize blood vessels, particularly in ophthalmology for retinal imaging.
Employed in diagnostics for tracing and imaging of tissues and fluids.
2. Fluorescence Microscopy and Assays
A key fluorescent probe in various immunofluorescence and fluorescence in situ hybridization (FISH) techniques.
Used in enzyme-linked immunosorbent assays (ELISA) and other fluorescence-based bioassays due to its high sensitivity.
3. Environmental and Industrial Applications
Utilized as a tracer dye for detecting leaks and monitoring fluid flow in water systems, pipelines, and environmental studies.
Helps in tracking pollutant dispersion in water bodies.
4. pH Indicator
Acts as a pH-sensitive fluorescent dye, useful in chemical and biological systems to monitor pH changes.
5. Educational and Research Uses
Commonly used in laboratory teaching for demonstrating fluorescence.
Serves as a standard in spectrofluorometry and fluorescence calibration.
Benefits
1. High Fluorescence Intensity
Exhibits bright and stable green fluorescence with a high quantum yield, enhancing sensitivity in detection.
2. Water Solubility
The disodium salt form ensures excellent water solubility, facilitating use in aqueous biological and environmental systems.
3. Photostability
Resistant to photobleaching, allowing prolonged observation in microscopy and imaging.
4. Low Toxicity
Generally considered non-toxic at working concentrations, making it suitable for live-cell imaging and clinical applications.
5. Versatility
Compatible with various chemical environments and conjugation techniques for labeling biomolecules.
Conclusion
Fluorescein disodium salt (CAS 518-47-8) is a widely used water-soluble fluorescent dye with applications spanning biomedical imaging, environmental tracing, and chemical assays. Its strong fluorescence, photostability, and versatility make it an indispensable tool in both research and applied sciences, particularly for sensitive detection and visualization in biological and environmental contexts.
