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Specifications
| Maximum absorption wavelength, λ (pH 8.0): | 490.0nm to 493nm |
| Mass absorption coefficient, λ (pH 8.0): | 200 L/cm·g min |
| Ethanol solubility test: | Conforms |
| Sensitivity test: | Conforms |
| Loss on drying: | 5% max |
| Residue on ignition (Calculated as sulfate): | 0.3% max |
Applications
1. Biological and Medical Imaging
Used extensively as a fluorescent tracer in microscopy, flow cytometry, and cell biology.
Used in ophthalmology for fluorescein angiography to visualize retinal blood vessels.
Serves as a diagnostic agent for detecting corneal abrasions and other eye injuries.
2. Fluorescence Microscopy and Assays
A key fluorescent dye for labeling cells, proteins, and nucleic acids.
Used in immunofluorescence, fluorescence in situ hybridization (FISH), and other fluorescence-based techniques.
3. Environmental Tracer
Used as a tracer dye in hydrology and environmental studies to track water flow, leaks, and pollutant dispersion.
4. pH Indicator
Acts as a pH-sensitive fluorescent dye in chemical and biological systems.
5. Educational and Research Tool
Common in laboratory teaching to demonstrate fluorescence and for instrument calibration.
Benefits
1. Bright and Stable Fluorescence
Exhibits bright green fluorescence with a high quantum yield.
Relatively photostable for many applications.
2. Versatility
Can be modified chemically or used in conjugation with biomolecules.
Compatible with aqueous and organic solvents.
3. Low Toxicity
Considered safe for many biological applications at working concentrations.
Conclusion
Fluorescein (CAS 2321-07-5) is a highly useful fluorescent dye applied in biomedical imaging, environmental tracing, and fluorescence assays. Its bright green fluorescence, ease of use, and broad applicability make it a staple in scientific research and diagnostics.
