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Specifications
| Appearance | White crystalline powder |
| Assay | 99% min |
| Moisture | 0.5% max |
| Loss on drying | 1% max |
| Solubility (1.0M aqueous) | Colorless, clear |
| PH value (1.0M aqueous) | 4.0 to 6.0 |
| Ultraviolet absorbance/260nm | 0.05 max |
Applications
1. Biological and Biochemical Buffer
Bicine is one of the Good's buffers, widely used in biological, biochemical, and clinical laboratories.
Commonly used in:
Enzyme assays (especially for enzymes active at slightly alkaline pH)
Cell culture and tissue samples
Protein and nucleic acid work
Electrophoresis buffer systems
2. Electrophoresis and Molecular Biology
Frequently used in polyacrylamide gel electrophoresis (PAGE) and other DNA/RNA or protein separation techniques.
3. Diagnostic and Pharmaceutical Reagents
Bicine is used in the formulation of diagnostic kits, IV fluids, and stabilizers in pharmaceutical solutions requiring alkaline buffering.
4. Research in Photosynthesis
Suitable for chloroplast and mitochondrial studies due to its compatibility with sensitive organelles and low metal chelation.
Benefits
✅ Effective Alkaline Buffer
pKa near 8.35 makes Bicine ideal for experiments requiring slightly basic pH control, such as in enzymology and biochemical assays.
✅ Low UV Absorbance
Minimal interference in UV detection (260–280 nm), suitable for spectrophotometric analyses.
✅ Low Metal Ion Binding
Minimal chelation of divalent cations like Mg²⁺ or Ca²⁺, ensuring compatibility with metal-dependent enzymes.
✅ Water Solubility
Easily dissolves in water, facilitating fast and consistent buffer preparation.
✅ Biological Compatibility
Non-toxic and non-interfering with most biological systems, making it safe for sensitive cells and enzymes.
Conclusion
Bicine (CAS 150-25-4) is a versatile, water-soluble biological buffer widely used in biochemical, molecular biology, pharmaceutical, and clinical applications, especially in systems requiring slightly basic pH control. Its stability, solubility, low interference, and compatibility with biological molecules make it a valuable tool in modern life science research and formulations.
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