BES Sodium Salt丨CAS 66992-27-6

BES Sodium Salt丨CAS 66992-27-6
Product Introduction:
Catalog No.: SS129970
CAS No.: 66992-27-6
Assay: 99% to 101%
Product Name: BES sodium salt
Molecular Formula: C6H14NNaO5S
Molecular Weight: 235.23
Synonym(s): N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt
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Technical Parameters
Description

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Specifications

 

Appearance White crystalline powder
Assay 99% to 101%
Water 0.5% max
Loss on drying 1% max
Solubility (1.0M aqueous) Colorless, clear
pH (1.0M aqueous) 6 to 8
Pb 0.0005% max
Ultraviolet absorbance (260nm) 0.05% max
Ultraviolet absorbance (280nm) 0.03% max
Applications

 

1. Biological Buffering Agent

BES sodium salt is a member of Good's buffers, used for pH stabilization in biochemical and biological experiments.

Commonly used in:

Cell culture media

Protein purification and enzymatic assays

Molecular biology techniques

2. Pharmaceutical Research and Formulations

Used in drug formulation buffers to maintain stable pH in biological preparations.

3. Electrophoresis

Suitable for use in electrophoretic separations where buffering near neutral pH is required.

4. Biochemical and Diagnostic Reagents

Incorporated into diagnostic assays and biochemical tests for pH control and reaction stability.

 

Benefits

 

1. Effective Buffering at Near-Neutral pH

Provides robust buffering capacity in the physiological pH range (~6.5 to 7.9), making it suitable for many biological systems.

2. Low Metal Ion Interaction

Minimal binding to metal ions such as Mg²⁺ and Ca²⁺, which is crucial for enzyme activity and cellular processes.

3. High Water Solubility

Easily dissolved in aqueous solutions, facilitating preparation of buffers.

4. Chemical and Thermal Stability

Stable under various experimental conditions, ensuring consistent performance.

5. Low UV Absorbance

Does not interfere with UV-based assays, allowing accurate spectrophotometric measurements.

 

Conclusion

 

BES sodium salt (CAS 66992-27-6) is a reliable and effective biological buffer for maintaining stable pH near neutrality. Its excellent solubility, minimal interaction with metal ions, and stability make it valuable in biochemical research, pharmaceutical development, electrophoresis, and diagnostic applications. BES supports reproducible and interference-free results across a broad spectrum of life science and clinical settings.

 

 

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