![3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic Acid丨CAS 68399-80-4](/uploads/42644/3-n-n-bis-2-hydroxyethyl-amino-2-hydroxy-1df6e1.png)
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Specifications
| Appearance | White crystalline powder |
| Assay | 99%~101% |
| Water | 0.5% max |
| Loss on drying | 1% max |
| Melting point | 189~192℃ |
| Solubility (1.0M aqueous) | Colorless, clear |
| pH (1.0M aqueous) | 3.0-5.0 |
| Heavy metal (Pb) | 0.0005% max |
| Ultraviolet absorbance/260nm | 0.05 max |
| Ultraviolet absorbance/280nm | 0.03 max |
Applications
1. Biological and Biochemical Buffering Agent
TES is a Good's buffer, widely used in biological and biochemical research to maintain stable pH environments near physiological conditions.
Commonly used in:
Cell culture media
Enzyme assays and protein purification
Molecular biology protocols requiring precise pH control
2. Electrophoresis
Utilized as a buffering component in polyacrylamide gel electrophoresis (PAGE) and other electrophoretic techniques to enhance resolution and reproducibility.
3. Pharmaceutical and Diagnostic Applications
Employed in formulation buffers for pharmaceutical products and diagnostic reagents, providing consistent pH stability during production and storage.
4. Cell Biology and Tissue Engineering
Supports cell viability and growth by buffering culture media without toxicity or interference with biological processes.
Benefits
1. Effective pH Buffering in Physiological Range
TES buffers efficiently around neutral pH, ideal for maintaining environments compatible with most biological systems and enzymes.
2. Low Metal Ion Binding
Exhibits minimal binding to divalent cations like Mg²⁺ and Ca²⁺, reducing interference with metal-dependent enzymes or cellular processes.
3. Chemical Stability
Maintains buffering capacity over a range of temperatures and storage conditions without significant degradation.
4. Low UV Absorbance
Transparent in UV and visible spectrum, making it suitable for spectrophotometric and fluorescence assays.
5. Biocompatibility
Non-toxic and non-interfering with cellular metabolism, making it excellent for in vitro and in vivo applications.
Conclusion
3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid (TES, CAS 68399-80-4) is a versatile biological buffer that provides stable pH control near physiological levels. Its excellent solubility, low metal binding, and biocompatibility make it a preferred choice for biochemical assays, cell culture, electrophoresis, and pharmaceutical formulations, supporting reliable and reproducible results in life sciences and medical research.
