Technical Parameters
Description
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High Purity Tween 80丨CAS 9005-65-6
Specifications
| Grade | USP / EP |
| Appearance | Oily, colorless or brownish-yellow, clear or slightly opalescent liquid |
| Solubility | Dispersible in water, anhydrous ethanol, ethyl acetate, and methanol; practically insoluble in fatty oils and liquid paraffin |
| Relative Density | 1.06–1.09 (about 1.10) |
| Viscosity | 300–500 mm²/s (about 400 mPa·s) |
| Acid Value | ≤2.0 |
| Saponification Value | 45–55 |
| Hydroxyl Value | 65–80 |
| Peroxide Value | ≤10 |
| Identification (1) | The infrared spectrum of the product is consistent with the control spectrum. |
| Identification (2) | Meets the requirements of the test for composition of fatty acids. |
| Identification (3) | The solution becomes blue. |
| Ethylene Oxide | ≤0.0001% |
| Dioxane | ≤0.001% |
| Water | ≤3.0% |
| Acidity or Alkalinity | 4.0–7.5 |
| Ethylene Glycol | ≤0.01% |
| Diethylene Glycol | ≤0.01% |
| Residue on Ignition | ≤0.25% |
| Total Ash | ≤0.25% |
| Myristic Acid | ≤5.0% |
| Palmitic Acid | ≤16% |
| Palmitoleic Acid | ≤8.0% |
| Stearic Acid | ≤6.0% |
| Oleic Acid | ≥58% |
| Linoleic Acid | ≤18.0% |
| Linolenic Acid | ≤4.0% |
| Bacterial Endotoxin | ≤0.012 EU/mg |
| Total Aerobic Microbial Count (TAMC) | ≤100 CFU/mL |
| Total Yeast and Mold Count (TYMC) | ≤100 CFU/mL |
| Escherichia coli | Absent |
| Pseudomonas aeruginosa | Absent/g |
| Salmonella spp. | Absent/10 g |
| Staphylococcus aureus | Absent/g |
Chemical Identifiers
| InChI | InChI=1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+ |
| InChI Key | RGPBUVUVZKQNHD-MDZDMXLPSA-N |
| Smiles | CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO |
| EC No. | |
| MDL No. | MFCD00082107 |
| PubChem CID | 5284448 |
Applications
Pharmaceutical Industry
Tween 80 is widely used as an emulsifier, solubilizer, and stabilizer in pharmaceutical formulations, including injectable preparations, oral formulations, and topical products. It can help improve the dispersion and stability of poorly water-soluble ingredients.
Biopharmaceutical & Life Science
It is used in cell culture media, protein formulations, vaccines, and bioprocessing applications. Tween 80 can help reduce surface adsorption and protect sensitive biological molecules during processing and storage.
Cosmetics & Personal Care
Tween 80 is commonly used in creams, lotions, cleansers, shampoos, and other personal care products as an emulsifier and solubilizer, helping maintain uniform and stable formulations.
Industrial & Laboratory Applications
Tween 80 is also used as a nonionic surfactant in detergents, coatings, laboratory formulations, and research applications where effective wetting, emulsification, or solubilization is required.

