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Specifications
| Appearance: | White to off-white powder |
| Solubility Clarity: | 0.2 M solution in acetonitrile is free from undissolved particulate |
| Identity (by HNMR): | To conform structure |
| Purity (HPLC): | 99.0% min |
| Single critical impurity: | 0.3% max |
| Non-critical single impurity: | 0.5% max |
| Unknown single impurity: | 0.3% max |
| 31P NMR Purity: | 98.0% min |
| P(III) Impurities (100–170 ppm): | 0.5% max |
| Residual Solvent: | 3.0% max |
| Moisture: | 0.5% max |
Applications
1. Synthesis of Modified Oligonucleotides
Key reagent for incorporating 2′-F-dC into DNA or RNA-like oligonucleotides.
Used in antisense oligonucleotides (ASOs), siRNA, aptamers, and other therapeutic or research RNA constructs.
2. RNA Therapeutics Development
2′-F-dC enhances resistance to enzymatic degradation, extends RNA half-life in vivo, and supports target engagement.
Often used in partially or fully 2′-modified oligonucleotide backbones to fine-tune biological properties.
3. RNA Mimicry in Research
Supports structural and biochemical studies involving RNA-like molecules.
Helps probe RNA-protein interactions, RNA folding, and hybridization dynamics.
4. Diagnostics and Probes
Ideal for designing nucleic acid probes used in qPCR, hybridization assays, and molecular diagnostics that require high thermal stability and specificity.
Benefits
✔️ High Nuclease Resistance
The 2′-fluoro substitution significantly increases resistance to ribonucleases, crucial for in vivo applications.
✔️ Enhanced Duplex Stability
Increases melting temperature (Tm) and improves target binding affinity in hybrid duplexes.
✔️ Reduced Immunogenicity
2′-F modifications minimize activation of innate immune receptors, making them safer for therapeutic development.
✔️ Efficient Synthesis Compatibility
Protective groups ensure clean, high-yield coupling and compatibility with standard phosphoramidite chemistry.
✔️ Versatile Use
Useful in both DNA-like and RNA-like oligonucleotide design, giving flexibility to fine-tune backbone chemistry.
Conclusion
5-O-DMT-2′-Fluoro-dC(N-Ac)-3′-CEDPA (CAS 159414-99-0) is a high-performance phosphoramidite for introducing 2′-fluoro-modified cytidine into synthetic oligonucleotides. It enhances biological stability, hybridization strength, and reduces immunogenicity, making it an essential reagent in the development of RNA-based therapeutics, antisense technologies, and diagnostic probes. Widely used in nucleic acid drug discovery and functional RNA research, it supports the creation of next-generation, stable, and effective oligonucleotides.
