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Specifications
| Appearance | White Powder |
| Assay (Titration, Dry basis) | 99% min |
| Water Content | 3.0% max |
| Infrared | Complies |
| pH (1% Di H2O, 25°C) | 6.7 to 7.3 |
| Solubility (0.1M in water) | Clear, Colorless Solution |
| A280 (0.1M in water) | 0.050 max |
| A260 (0.1M in water) | 0.050 max |
| Lead (Pb) | 5 ppm max |
Applications
1. Biological and Biochemical Buffer
MOPS hemisodium salt serves as an effective Good's buffer for stabilizing pH in biological and biochemical experiments near neutral pH.
Commonly used in:
Cell culture media
Enzyme assays
Protein purification
Molecular biology protocols including electrophoresis
2. Pharmaceutical and Diagnostic Formulations
Utilized in pharmaceutical preparations and diagnostic reagents requiring tight pH control around neutrality.
3. Research and Industrial Applications
Used for pH stabilization in biochemical studies and industrial bioprocesses where precise pH maintenance is critical.
Benefits
✅ Optimal Buffering Near Neutral pH
Provides effective pH control around physiological pH with minimal fluctuations.
✅ Low Metal Ion Binding
Exhibits minimal interaction with divalent metal ions, preserving enzymatic activity and cofactor availability.
✅ High Water Solubility
Easily dissolves in water, allowing convenient and reproducible buffer preparation.
✅ Chemical Stability
Stable under laboratory and industrial conditions, ensuring consistent experimental results.
✅ Low UV Absorbance
Compatible with UV spectrophotometry, minimizing interference in assays involving nucleic acids or proteins.
Conclusion
MOPS hemisodium salt (CAS 117961-20-3) is a valuable buffering agent for maintaining stable pH near neutrality in biochemical, pharmaceutical, and molecular biology applications. Its solubility, stability, and low interference with biological molecules make it a reliable choice for researchers and industry professionals requiring precise pH control.

