Hepps丨CAS 16052-06-5

1. Biological and Biochemical Buffer

HEPPS is a Good's buffer, widely used in biochemical and molecular biology research for maintaining pH stability near physiological alkaline conditions.

Common applications include:

Enzyme assays

Protein purification

Cell culture media

Electrophoresis and other molecular biology techniques

2. Pharmaceutical and Diagnostic Formulations

Used in formulations requiring stable pH around 8, such as in certain drug preparations and diagnostic reagents.

3. Biotechnological Research

Useful in studies of biochemical processes where enzyme or protein activity is optimal at mildly alkaline pH.

✅ Effective Buffering in Slightly Alkaline Range

pKa around 8.0 provides excellent buffering near physiological pH levels for many biological processes.

✅ Low Metal Ion Binding

Minimal interference with divalent cations (Mg²⁺, Ca²⁺), preserving enzyme function.

✅ High Water Solubility

Easily dissolved in aqueous solutions for convenient buffer preparation.

✅ Chemical Stability

Stable under various laboratory conditions, ensuring reliable and reproducible results.

✅ Low UV Absorbance

Compatible with spectrophotometric assays without affecting absorbance measurements.

HEPPS (CAS 16052-06-5) is a highly effective biological buffer ideal for maintaining stable pH in the slightly alkaline range (7.6–8.2). Its stability, low metal ion interaction, and compatibility with biological systems make it valuable in biochemical research, pharmaceutical formulation, and molecular biology applications requiring precise pH control.