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Specifications
| Appearance | White crystalline powder |
| Assay | 99% min |
| Loss on drying | 1% max |
| Solubility | Colorless, transparent |
Applications
1. Biological and Biochemical Buffer
HEPES potassium salt is widely used as a buffering agent in biological research, especially for maintaining physiological pH in cell culture media.
Supports enzyme activity assays, protein purification, and molecular biology experiments.
2. Cell Culture
Provides stable pH environment for mammalian and microbial cell cultures, often used in CO₂-independent buffering systems.
3. Pharmaceutical Formulations
Used in drug formulations where pH stability is crucial, including injectable drugs and diagnostic reagents.
4. Electrophoresis and Biochemical Assays
Employed in PAGE, Western blotting, and other electrophoretic techniques due to its stable buffering near neutral pH.
Benefits
1. Stable pH Buffering Near Physiological Range
With a pKa near 7.5, it effectively maintains pH around 7.2–7.6, ideal for many biological systems.
2. Minimal Interference with Biological Processes
Exhibits low metal ion binding and UV absorbance, making it compatible with a wide range of assays.
3. High Water Solubility
Easily dissolved in aqueous solutions, allowing simple buffer preparation.
4. Biocompatibility and Low Toxicity
Safe for use with live cells and sensitive enzymatic systems.
Conclusion
HEPES potassium salt (CAS 82207-62-3) is a highly effective and widely used biological buffer ideal for maintaining stable physiological pH in diverse applications including cell culture, biochemical assays, pharmaceutical formulations, and electrophoresis. Its chemical stability, biocompatibility, and low interference make it a staple in modern life science research and industry.
