N-Succinimidyl Maleimidoacetate丨CAS 55750-61-3

N-Succinimidyl maleimidoacetate is widely used as a bifunctional crosslinking reagent in bioconjugation, protein modification, and biomolecular research. It contains both an N-hydroxysuccinimide (NHS) ester and a maleimide group, allowing selective conjugation to primary amines and thiol groups, respectively. This makes it ideal for linking peptides, proteins, antibodies, or other biomolecules in a controlled manner. The compound is also employed in the preparation of targeted drug delivery systems, fluorescent probes, and diagnostic reagents, where stable and site-specific attachment of functional molecules is critical. Additionally, it serves as a tool in chemical biology and proteomics for studying protein interactions and labeling specific biomolecular sites.

The benefits of N-Succinimidyl maleimidoacetate include its high reactivity, selectivity, and ability to form stable covalent linkages under mild conditions. The NHS ester reacts efficiently with amino groups, while the maleimide selectively targets thiols, enabling precise dual-functional modification. Its chemical stability in dry form and predictable reactivity in aqueous or organic media support reproducible conjugation reactions. This dual functionality reduces unwanted side reactions, improves labeling efficiency, and allows controlled orientation of conjugated biomolecules, making it a practical reagent for research and therapeutic applications.