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Specifications
|
Appearance |
White crystals |
|
Assay (on dried basis) |
98% min |
|
Water insoluble |
0.01% max |
|
Chloride (Cl) |
0.005% max |
|
Sulfate (SO4) |
0.005% max |
|
Iron (Fe) |
0.001% max |
|
Heavy metals (Pb) |
0.001% max |
|
pH |
6.5 – 7.5 |
Applications
1. Buffering Agent in Biochemistry
Ammonium acetate丨CAS 631-61-8 is widely used as a buffer in biological and biochemical applications, especially in:
Mass spectrometry (MS)
High-performance liquid chromatography (HPLC)
Capillary electrophoresis
It maintains mildly acidic to neutral pH when dissolved in water, making it suitable for DNA and protein handling.
2. Mass Spectrometry and HPLC
In LC-MS and HPLC, ammonium acetate is valued for being:
Volatile, leaving no residue after evaporation
Non-ionic in solution, helping prevent interference with detection
It's commonly used as a mobile phase additive to improve peak shape and signal clarity.
3. Reagent in Organic Chemistry
Ammonium acetate serves as a reagent or catalyst in:
Acetylation reactions
Strecker synthesis of amino acids
Reductive amination
As a source of ammonia in reactions that require in situ generation of NH₃
4. Textile and Dye Industry
Used in dye formulations and fabric treatments as a buffering or stabilizing agent to control pH and enhance dye uptake.
5. Fertilizer and Agriculture
Although not a common agricultural fertilizer, ammonium acetate can be used:
As a nitrogen source in specialty formulations
In some soil treatment research
6. Food Additive (E264)
In some regions, ammonium acetate is allowed as a food additive:
Acts as an acidity regulator
Not widely used in food due to limited flavor compatibility, but still permitted in specific applications
7. Analytical Chemistry
Used in Karl Fischer titration and qualitative analysis
Employed in preparing reference buffers or sample diluents
Benefits
✅ 1. Volatility
Ammonium acetate is thermally volatile, making it ideal for mass spectrometry and chromatographic techniques where non-volatile salts would contaminate equipment or interfere with detection.
✅ 2. Dual-Function Buffer
Acts as both a weak acid (acetic acid) and a weak base (ammonia).
Provides excellent buffering capacity around pH 4.5–6.0, suitable for many enzymatic and nucleic acid processes.
✅ 3. Biocompatibility
Gentle and non-denaturing, it is commonly used in nucleic acid precipitation, extraction, and purification processes.
Compatible with enzymes and cells in biological preparations.
✅ 4. Readily Available and Cost-Effective
Inexpensive, stable, and easy to handle, making it a lab staple for analytical, preparative, and industrial workflows.
✅ 5. Clean Reaction Profile
In organic synthesis, its decomposition products (acetic acid and ammonia) are easy to remove, leaving fewer byproducts and simplifying purification.
Summary
Ammonium acetate丨CAS 631-61-8 is a versatile buffering agent, analytical reagent, and organic chemistry intermediate. Its volatility, biocompatibility, and cost-effectiveness make it indispensable in applications like mass spectrometry, HPLC, protein/DNA analysis, and synthetic chemistry. Due to its mild pH and easy breakdown into benign components, it's preferred in sensitive workflows requiring minimal background contamination.
