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Specifications
| Appearance | Colorless crystals or white crystalline powder |
| Identification (¹H NMR) | Conforms to the structure |
| Purity (HPLC) | 99% min |
| Free 4-Methylumbelliferone | 25 ppm max |
| Specific Optical Rotation [α]D20 (c = 0.25, H₂O) | -103.0° to -107.0° |
| Solubility (1% in water at 60 °C) | Clear, colorless solution |
| Water Content (Karl Fischer) | 8.0% – 10.2% |
Applications
4-Methylumbelliferyl-β-D-glucuronide (CAS 6160-80-1) is a fluorogenic substrate widely used in biochemical, clinical, and microbiological assays. It is primarily applied for the detection and quantitative measurement of β-glucuronidase activity, an enzyme relevant in medical diagnostics, pharmaceutical research, and environmental testing. Upon enzymatic hydrolysis, it releases 4-methylumbelliferone, a strongly fluorescent compound, making it valuable in enzyme kinetics studies, drug metabolism research, and screening of enzyme inhibitors. The compound is also used in microbiology to identify β-glucuronidase-positive microorganisms and in toxicology and pharmacology to evaluate glucuronidation and deconjugation pathways. Its sensitivity and specificity make it suitable for high-throughput assays and fluorescence-based analytical methods.
Benefits
The benefits of 4-methylumbelliferyl-β-D-glucuronide come from its high sensitivity, specificity, and ease of detection. The fluorogenic response allows rapid and accurate measurement of β-glucuronidase activity at very low enzyme concentrations. Its well-defined structure ensures reproducible enzymatic cleavage and consistent fluorescence output, supporting reliable quantitative analysis. The compound is compatible with standard fluorescence detection equipment and assay formats, enabling straightforward integration into laboratory workflows. Additionally, its stability under normal storage conditions and solubility in common assay buffers make it practical for routine use in research, clinical, and quality control laboratories.
Conclusion
4-Methylumbelliferyl-β-D-glucuronide is a highly effective fluorogenic substrate for detecting and quantifying β-glucuronidase activity. Its sensitivity, specificity, and compatibility with fluorescence-based assays support applications in diagnostics, microbiology, toxicology, and pharmaceutical research. By enabling accurate and efficient enzyme analysis, it remains an essential tool in biochemical and analytical science.
