6-HEX丨CAS 155911-16-3

6-HEX (CAS 155911-16-3) is a synthetic fluorescent dye primarily used in molecular biology and analytical chemistry. Its main applications include labeling DNA and RNA sequences for detection in fluorescence-based techniques such as quantitative PCR and fluorescence in situ hybridization (FISH). It is also incorporated into fluorescent primers to monitor nucleic acid amplification in real time. Additionally, 6-HEX is used in gene expression studies to visualize specific gene activity within tissues or cell populations, as well as in biosensors for detecting biomolecules or enzymatic activities. Its excitation and emission properties make it suitable for high-sensitivity detection and multiplexed assays.

The benefits of 6-HEX are centered on its high fluorescence intensity, stability, and compatibility with biological systems. It provides strong signal output even at low concentrations, enabling sensitive and accurate detection of nucleic acids and other biomolecules. Its defined excitation (532 nm) and emission (556 nm) maxima allow it to be used alongside other fluorescent dyes in multiplex assays without significant spectral overlap. Additionally, its chemical stability ensures consistent performance during storage and experimental procedures, while its solubility in organic solvents and moderate aqueous solubility provide flexibility in various assay conditions. These characteristics make 6-HEX an efficient and reliable tool for advanced molecular biology and analytical applications.