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Specifications
| Appearance: | White granular |
| Exchange capacity (on dried basis): | 1.0±0.1 mmol/g |
| Water: | 18% max |
| Protein Load (BSA, pH 8.5): | 100 min |
Applications
1. Ion-Exchange Chromatography
DEAE-cellulose is widely used as a weak anion exchanger in ion-exchange chromatography, a key technique in:
Protein purification
Enzyme separation
Nucleic acid (RNA/DNA) isolation
Antibody purification
The DEAE group introduces positive charges, which bind negatively charged molecules (like DNA, RNA, acidic proteins).
2. Biotechnology and Biopharmaceutical Manufacturing
Used in downstream processing of biologics such as monoclonal antibodies, vaccines, recombinant proteins, etc.
Plays a crucial role in GMP-compliant purification of therapeutic proteins and enzymes.
3. Research and Laboratory Use
Essential tool for biochemical separation techniques in molecular biology, proteomics, and genomics.
Commonly used in academic labs for column chromatography of nucleic acids and proteins based on charge.
4. Diagnostic and Analytical Applications
Used in diagnostic kits or experimental setups requiring ion-exchange capacity, such as:
Serum protein fractionation
Isoenzyme separation
Plasma component analysis
Benefits
1. High Selectivity and Resolution
Excellent separation of biomolecules based on isoelectric point (pI).
Allows for stepwise or gradient elution, enhancing purity of target compounds.
2. Biocompatibility
Derived from cellulose, making it non-toxic, biodegradable, and suitable for biological applications.
Does not denature or degrade sensitive biomolecules like enzymes or antibodies.
3. Adjustable Binding Strength
As a weak base anion exchanger, DEAE-cellulose works over a broad pH range, allowing control over binding and elution conditions.
Binding can be easily disrupted using salt gradients or pH shifts.
4. Cost-Effective and Reusable
Can be regenerated and reused multiple times in laboratory workflows.
Economical for both research and industrial-scale operations.
5. Customizable Formats
Available as fibrous, granular, or microgranular materials.
Suitable for both batch and column chromatography.
Conclusion
DEAE-Cellulose (CAS 9013-34-7) is a widely used anion exchange resin derived from cellulose. Its primary application lies in biomolecular purification, especially for proteins and nucleic acids. It is favored for its biocompatibility, tunable binding properties, and cost-effectiveness, making it indispensable in life sciences research, biotech manufacturing, and clinical diagnostics.
