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Specifications
| Appearance | Tan to orange powder |
| pH Visual Transition Interval | 3.0 (yellow) to 4.6 (bluish violet) |
| Mass Absorptivity (L/cm·g) | 115 min |
| Solubility in Ethanol | Conform |
| Ignition Residue | 0.25% max |
Applications
Bromophenol Blue is a well-known pH indicator dye widely used in analytical chemistry, molecular biology, and laboratory diagnostics. It functions primarily as a pH indicator, shifting color between yellow and blue in the approximate range of pH 3.0 to 4.6, which makes it useful in titrations and buffer preparation. In biochemistry, it is commonly employed as a tracking dye in gel electrophoresis, helping researchers monitor the progress of nucleic acid and protein separation. It also finds use in microbiology as a visual marker in culture media and diagnostic assays. Additionally, Bromophenol Blue is applied in industrial quality control processes, particularly in formulations requiring pH monitoring or as a dye additive.
Benefits
The benefits of Bromophenol Blue arise from its strong color contrast, versatility, and stability under a variety of conditions. Its sharp color transition within a defined pH range makes it a reliable indicator for both educational and research laboratories. As a tracking dye, its migration properties in electrophoresis gels provide researchers with a convenient, non-interfering method to monitor sample progress without affecting molecular integrity. The compound is cost-effective, easy to use, and compatible with many biological and chemical systems, enhancing efficiency in analytical work. Its reproducibility and versatility across chemical, biochemical, and industrial applications further strengthen its value as an essential laboratory reagent.
Conclusion
In summary, Bromophenol Blue is a multifunctional dye with important roles as a pH indicator, electrophoresis tracking dye, and diagnostic marker. Its benefits include clear color transitions, compatibility with biochemical systems, cost-effectiveness, and stability. By combining analytical precision with practical versatility, Bromophenol Blue continues to be an indispensable reagent in scientific research, education, and industrial applications.
